KMID : 0370220060500060358
|
|
Yakhak Hoeji 2006 Volume.50 No. 6 p.358 ~ p.366
|
|
Hepatoprotective Activities of Glycyrrhizin and Baicalin in Primary Cultured Rat Hepatocytes
|
|
Kim Sung-Hwa
Kang Sam-Sik Cheon Ho-Jun Park Jin-Gu Kim Yeong-Shik Xu Guang-Hua Lee Seung-Ho Son Kun-Ho Lee Sun-Mee
|
|
Abstract
|
|
|
The aim of this study was to investigate the protective effects of glycyrrhizin, active glycosides of Glycyrrhizae Radix, and baicalin, bioactive flavonoid isolated from Scutellariae Radix, on hepatocyte injury induced by carbon tetrachloride(CCl4, 10mM), tert-butyl hydroperoxide (TBH, 0.5 mM), and D-galactosamine (GalN, 30mM). Primary cultures of rat hepatocyte (18 hr cultured) were treated with CCl4, TBH, or GalN and various concentrations (0.1, 1, 10, and 100 ¥ìM) of glycyrrhizin or baicalin. Activity was accessed by determining the release of lactate dehydrogenase (LDH) and aminotransferses. CCl4 significantly increased the levels of LDH, alaine aminotransferase (ALT), and aspartate aminotransferase (AST) and these increases were prevented by baicalin concentrations of 0.1, 1, and 100¥ìM. The increases in ALT and AST levels were reduced by glycyrrhizin concentration of 100 ¥ìM. The level of LDH was markedly increased by TBH, and this increase was reduced by both glycyrrhizin and baicalin. ALT and AST levels were increased by TBH, which were prevented by glycyrrhizin and bacalin, respectively. GalN markedly increased the levels of LDH, ALT and AST. These increases was significantly reduced by both glycyrrhizin and baicalin. These results suggest that glycyrrhizin and baicalin possess the hepatoprotective activity.
|
|
KEYWORD
|
|
glycyrrhizin, baicalin, hepatoprotective acivity, primary cultured hepatocyte
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|
|